棉 花 学 报      Cotton Science    2009，21(4)：324-329

Multiple-shoot Induction and Genetic Transformation of Island Cotton ( Gossypium barbadense L.) in Xinjiang
ZHOU Xiao-feng¹，JIN Shuang-xia²，LI Xiang²，LI Bao-cheng¹，IAN Wen-ming³，NING Xin-zhu¹，LIU Li¹
(1.Institute of Cotton, Xinjiang Academy of Agricultural Reclamation Sciences，Shihezi，Xinjiang 832000，China ；2.National Key Laboratory of Crop Genetic Improvement，Huazhong Agricultural University, Wuhan 430070，China ；3．Institute of Agriculture Sciences；Xinjiang Reclamation Second division，Alaer，Xinjiang 843000，China)

Abstracts：With three island cottons，Xinhai 17，Xinhai 14, 85H, shoot organogenesis and genetic transformation were studied in Xinjiang. The results were as follows: in vitro plant regeneration of meristem shoot tip is better, and the seedling rate of 90.5%was obtained. Frequency of inducing shoots reached maximum with 6-BA at 0.5 mg·L^-1; whereas number of budding shoots in per explant reached maximum with 6-BA at 1.5 mg·L^-1; plantlet from shoot apical meristem repressed with 6-BA at 2.0 mg·L^-1；The maximum budding shoots in per explant is four. Isolated meristem shoot tips infected with Agrobacterium tumefacien have strong endurance on infected damnification and kan, of which selective concentration reached 100 mg·L^-1. The rate of plantlet from infected shoot tips on selection medium reached to 88.9%。
Key words：island cotton; multiple-shoot inducement; genetic transformation    [Full Text, 4408KB]