棉 花 学 报     Cotton Science    2009，21(4)：279-283

Molecular Marker-assisted Selection and Pyramiding Breeding of Major QTLs for Cotton Fiber Length
DONG Zhang-hui，SHI Yu-zhen，ZHANG Jian-hong，WANG Shu-fang，LI Jun-wen，LIU Ai-ying，TANG Shu-rong，CHU Ping ，YUAN You-lu^*
(Cotton Research Institute of the Chinese Academy of Agricultural Sciences，Key Laboratory of Cotton Genetic Improvement，Ministry of Agriculture, Anyang，Henan 455000，China)

Abstract：TG41 and sGK156, two commercial cotton cultivars (lines), and HS427-10, 7235 and 0-153, three elite fiber quality germplasm lines, were used as parents to develop three double-cross combinations, (sGK156×HS427-10)×(0-153×7235), (TG41×HS427-10)×(0-153×7235) and (sGK156×0-153)×(sGK156×HS427-10).Three SSR markers, NAU1043, MUSS497 and CM67, which were linked with QTLs of fiber length and derived from 7235 or 0-153, were used to study the effect of molecular marker-assisted selection and pyramiding breeding in the three combinations, respectively. As a result, the fiber length showed significant differences between plants with and without marker in the three combinations. This indicated that the effects of the three QTLs for fiber length were stable in different genetic backgrounds and different generations. When selected the plants with two or three markers, the fiber length of plants with two or three markers were better than that of the plants with only one marker, and the fiber length was the best in the plants with all the three markers. In conclusion, it is effective to increase fiber length through molecular marker-assisted selection and the effect may be increased when more QTLs are pyramided in plants. It is necessary to develop stable recombinant inbred lines of advanced generations which pyramid several fiber-related genes to study the effect for pyramiding more genes.
Key words：cotton；fiber length；QTL；molecular marker；breeding     [Full Text, 2171KB]