棉 花 学 报      Cotton Science        2009，21(4)：275-278

Cloning and Expressing of Putative Enolase Gene from Cotton （Gossypium hirsutum L.）
WANG Yi-xue^1，3，SUN Yi^1，3，TIAN Ying-chuan²，WU Jia-he^2*
(1.College of Agriculture, Shanxi Agricultural University,Taigu,Shanxi 030801, China; 2.State Key Laboratory of Plant Genomics,Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101，China; 3.The Agri-Biotechnology Research Center of Shanxi Province, Taiyuan 030031, China)

Abstract：The total RNA of Gossypium hirsutum was extracted by modified CTAB method and the open reading frame of enolase gene from Gossypium hirsutum was cloned by RT-PCR，named GhEno, which was 1338 bp long and encoded 445 amino acids．The GhEno gene was registered in GenBank with the access number of EU169604．Sequence analysis alignment indicated that GhEno gene had high homology with those of other plants. The phylogenetic tree analysis showed that the GhEno was clustered in the same group with Hevea brasiliensis and Ricinus communis. Semi-quantity RT-PCR analysis indicated that expression of GhEno was detected in roots, stems and leaves， however it was stronger in roots than in leaves and stems.
Key words：cotton （Gossypium hirsutum L.）；enolase；gene cloning；gene expression
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