棉 花 学 报    Cotton Science    2009，21(4)：271-274

Influencing Factors of Induction Ratio of Resistant Callus by Agrobacterium- mediated Genetic Transformation in Cotton
ZHAO Chang-yan，WANG Xing-fen，HAN Gai-ying，CHI Ji-na，ZHANG Gui-yin，MA Zhi-ying^*
(Key Laboratory of Crop Germplasm Resources of Hebei /Agricultural University of Hebei, Baoding, Hebei 071001, China)

Abstract：The influences of Agrobacterium concentration,infection time,the time and the temperature of co-cultute on the induction ratios of kanamycin-resistant calli were studied by Agrobacterium -mediated transformation using the hypocotyls of Jiwu 2031 as explants.The factors were optimized using orthogonal design.The results showed that the transformation ratio was the highest under the regime of OD=0.3 of bacteria concentration,15 min of infection time, 24 ℃ of co-culture temperature and 24 h of co-culture time.High frequency of kanamycin-resistant calli formation was observed when infecting hypocotyls of Coker 312,Zhong 521 and Nongda 94-7 using the optimized combination of factors,and the induction ratios reached 93.85%,86.72% and 85.83%,respectively.
Key words：cotton; Agrobacterium- mediated; orthogonal design; kanamycin-resistant callus
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