棉 花 学 报      Cotton Science    2009，21(3)：236－242

Cloning and Construction of GhcelA 1 Gene in Cotton Fiber
FAN Xiao-ping^1，2，FAN Bo-hong²，XU Zi-qin^1*，YANG Wei-cai^3*
(1.Colleage of Life Science，Northwest University，Xi′an 710069，China；2.Institute of Cotton Research，Shanxi Academy of Agricultural Sciences，Yuncheng，Shanxi 044000，China；3.Institute of Genetics and Developmental Biology，The Chinese Academy of Sciences，Beijing 100101，China)

Abstract：GhcelA 1 cDNA full length which reported in GeneBank (U58283) was cloned from Coker 312 ( Gossypium hirsutum ) fiber at 20 DPA (days after anthesis) for the first time using RT-PCR method. A serial of plant expression vectors for over-expression, fusion gene with EGFP (Enhanced Green Fluorescent Protein) and RNAi (interference) by recombination technology were constructed and confirmed by molecular and sequence methods. Expression of EGFP in infected cells was clearly evident in confocal microscopy (Zeiss LSM-510) experiments. This showed that the GhcelA 1-EGFP fusion in correct construction and could express in cotton cells successfully. Clone and construction were the precondition of transformation for culture improved variety and studying on the pivotal function of cellulose synthetase in the second cell wall thicken period of cotton fiber.
Key words：cotton；cellulose synthetase；gene clone；vector construct     ［Full Text, 6003KB］