ÃÞ »¨ ѧ ±¨ Cotton Science 2009£¬21(3)£º196-200
Construction and Application of Molecular Linkage Group of the 14th Chromosome of Cotton Abstract£ºDevelopment of each alien species chromosome substitution line in upland cotton involves three stages £º(a) development of the respective TM-1-like hypoaneuploid stock, (b)use of the cytogenetic stock as recurrent parent in a recurrent backcrossing program to create a monosomic or monotelodisomic substitution stock, followed by (c)inbreeding to recover a euploid disomic substitution line. Up to now, 17 CSB lines have been developed, in which a pair of chromosomes (or chromosome arms) of G. hirsutum inbred cultivar, TM-1, are replaced by corresponding pair of G. barbadense line, 3-79. It is important to determine the chromosome substitution lines introduced. CSB14Sh, which are nearly the same as their recurrent parent TM-1, except the 14th chromosome short arms, crossed with TM-1, and the F ª 2 population was produced. A total of 3800 SSR primer pairs covering whole genome were used to screen polymorphism between two parents, TM-1 and CSB14Sh, which resulted in 15 polymorphic primer pairs. These 15 pairs amplified 23 marker loci . Linkage test indicated 21 of 23 loci could be mapped to one linkage group and covered distance of 65.3 cM. Compared with the map constructed previously, the linkage group was mapped to the 14th chromosome. These results showed that the only difference between CSB14Sh and TM-1 is in the 14th chromosome. Considering to the development of chromosome substitution line and results above, CSB14Sh is the very 14th chromosome short arm of G. hirsutum inbred cultivar, TM-1.
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