棉 花 学 报    Cotton Science   2009，21(3)：184-189

Cloning and Analysis of Adenylyl Cyclase-associated Protein Genes in Gossypium arboreum L.
WANG Sheng，ZHAO Guo-hong，DU Xiong-ming^*
( Cotton Research Institute，Chinese Academy of Agricultural Sciences，Key Laboratory of Cotton Genetic Improrement，MOA，Anyang，Henan 455000，China)

Abstract：Adenylyl cyclase-associated proteins (CAPs)，functioning in cytoskeletal organization and signal transduction, may play an important role in cell development. Using PCR method, the DNA and cDNA sequences of CAP were isolated from Gossypium arboreum L.. The 1425 bp cDNA contains an open reading frame (ORF) encoding 471 amino acid residues. The encoding region had all four of the typical domains of CAP protein family. It was named GaCAP . The C-terminal domains could bind to actin protein. And a common feature of the CAP proteins is the presence of a CAP motif in their N-terminal domains. The motif is characterized by RLE repeats, which have the potential to form an amphipathic helix that mediates protein-protein interactions, the RLE repeats are known to be involved in the binding to adenylyl cyclase. These results suggested that CAP may play an essential role in cell differentiation.
Key words：Gossypium arboreum L.；CAP；gene cloning；sequence analysis    [Full Text, 2470KB]