棉 花 学 报     Cotton Science   2009，21(2)：153-155

Study of Rapid Detection of Transgene Sequences in Cottonseed Oil Using PCR
YUAN Jian-qin₁，TANG Zhong-wei¹，XU Dong-mei¹，LIU Jian-dong¹，GAO Bin-zhan²
（1.College of Life Science，Shanxi Agricultural University，Taigu, Shanxi 030801, China；2.Lucker Pharmaceutical Stock Factory，Taigu, Shanxi 030800, China）

Abstract：A simple and rapid-method which could extract DNA from cottonseed oil had been developed. A yield of 0.4 μ g DNA could be obtained from 15 mL oil. PCR amplification of sequences of CaMV35S, NOS, NPT Ⅱ , Cry 1A （c）, and an endogenous reference gene 18S rDNA in refined cottonseed oil was performed. Results showed that the sequence-specific fragments were amplified in the samples, demonstrating that the method could be applied for detection of transgene sequences. There are some DNA fragments in refined cottonseed oil, and PCR amplification can detect the foreign DNA.
Key words：cottonseed oil；transgene sequences；DNA extraction；PCR; detection study
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