棉 花 学 报     Cotton Science     2009，21(1)：10-16

Cloning and Characterization of GhCHS and GhCPI Which Related with Fiber Development in Gossypium hirsutum L.
ZHANG Yan-jie, ZHU Yi-chao, GUO Wang-zhen, ZHANG Tian-zhen^*
(National Key Laboratory of Corp Genetics & Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095，China)

Abstract：To isolate genes preferentially expressed during fiber elongation period, genechips were performed with wild line li₁li₁ and the Ligon lintless mutant line Li₁li₁segregated from the self-crossing progenies of the Ligon lintless mutant line Li₁li₁. Two differently expressed cDNA clones were separated from cotton fiber library of elite quality material 7235 during fiber elongation and the second wall synthesis by genechips with probes of the 4 DPA ovule-fiber compound from the wild line li₁li₁and the Ligon lintless mutant line Li₁li₁. RT-PCR analysis indicated the expression quantity of two genes - GhCHS (GenBank accession: EF643506) and GhCPI (GenBank accession: EF643507) were less in the wild line li₁li₁than that in the Ligon lintless mutant line Li₁li₁at 4 DPA. The result of Southern blotting indicated that there are two copies of both genes in the genome of upland cotton.
Key words：upland cotton; genechips; chalcone synthase; cysteine proteinase inhibitor
           [Full Text, 3703KB]