ÃÞ »¨ ѧ ±¨ Cotton Science 2008£¬20(6)£º418-424
Comparison of SSR Types and Abundance Differences between G.raimondii and G.arboreum Abstract£ºThe 39277 G.arboreum and 63588 G.raimondii ESTs from NCBI databases which were in a total sizes of 64.08 Mb (approximately 6.02% of total cotton genome) were respectively assembled by bioinformatical methods, and the differences of Simple Sequence Repeat (SSR) types and abundance between these two species were analyzed. The main results were shown as follow: The singleton ratios of G.arboretum were higher than those of G.raimondii in the assembled tentative consensus sequences; The interval distances of six SSR types which were from mononucleotide to hexa-nucleotide repeats in G.arboreum were farther than those of the six SSR types in G.raimondii , respectively; The percentages of the five repeat types which were from di- to hexa-nucleotides were higher in G.arboreum than that of the five repeat types in G.raimondii . However, the percentages of mononucleotide repeat type were lower in G.arboreum . Moreover, the advanced SSR motifs and abundance of mono-, tetra-, and hexa-nucleotides repeat types were also different between two species. Therefore, it is suggested that the primers designed according to the tetra ª² or more nucleotides repeat types can effectively identify the genome differences between G.arboreum and G.raimondii . The differential analysis of SSR types and abundance in the donor species for A and D sub-genome of the tetraploid species provides more useful information for SSR marker design and profound research of the origin and evolution of Gossypium species.
|