棉 花 学 报    Cotton Science    2008，20(5)：323－329

Cloning of the Gene GH18Rorf 392 Containing 26S rRNA Sequence from Cotton Restorer line
WU Qiao－wen，SONG Yang，ZHANG Rui，WANG Yuan，GUO San－dui^*
(1. Biotechnology Research Institute，the Chinese Academy of Agriculture Sciences，Beijing 100081，China；2. Beijing People' s Police College，Beijing 102202，China)

Abstract：Y18R×P 30A hybrid F₁ generation then inbred, so judge the fertility according to stamen morphological character. Total RNA were extracted from young buds of 3 ～ 5 days from fertile and sterile cotton, respectively. EST sequence from cotton restorer line was obtained through suppression subtractive hybridization. Designing primers according to the EST sequence, then doing rapid amplification cDNA ends, so full length cDNA sequence was obtained. The gene was named by GH18Rorf 392 because it came from Gossypium hirsutum restorer line Y18R and codes 392 amino acids. The 5' end of GH18Rorf392 is a new sequence and its 3' end contains 26S rRNA partial sequence. This gene can be used to study cotton fertility associated function.
Key words：cotton restorer line；SSH；RACE；CMS；fertility restore     [Full Text, 4005KB]