棉 花 学 报       Cotton Science     2008,20(4):256-263

 

 

 

Introgression of Gossypium klotzschianum Genome into Cultivated Cotton, G. hirsutum
SHEN Xin-lian,ZHU Jing, ZHANG Xiang-gui, ZHANG Bao-long, CAO Zhi-bin, YANG Yu-wen, XU Peng, XU Ying-jun, NI Wan-chao
Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Abstract:Exotic Gossypium germplasms are rich sources of useful agronomic traits for improvement programs of cultivated cotton. Efficient use of genetic variation available in the wild relatives depends on the ability to identify and introgress desirable DNA segments from wild species into cultivated cotton. To introgress genes from G. klotzschianum into G.hirsutum,a BC1F2 population from (G.hirsutum×G.klotzschianumG.hirsutum was constructed. A total of 320 SSR primers covering the cotton genome were selected to monitor alien germplasm transmission. Only 38 markers showed segregation in the BC1F2 population, and they were distributed in 14 different chromosomes and constituted 18 introgressed segments. The total length of the 18 introgressed segments was 595 cM based on a cotton consensus map. Two morphological traits(yellow petal and open bud)were mapped on chromosome 13. Our molecular and morphological identification confirmed the presence of G. klotzschianum chromatin in the BC1F2 interspecific progenies. The use of species-specific molecular markers will greatly facilitate introgression of desirable alien genes into cultivated cotton.
Key words:G.klotzschianum;SSR;introgressed segment;species-specific molecular markers
CLC number:S562.035     Document code:A

Article ID:1002-7807(2008)04-0256-08     [Full Text, 3232KB]