Original£ºCotton Science£¬2006£¬18(3)£º131-134
Generation of Antibiosis-free Mutants of Bacillus subtilis NCD-2 Abstract£ºThe Bacillus subtilis NCD-2, isolated from the rhizosphere of cotton, could control cotton Verticillium wilt effectively in field. This bacterial strain inhibited the phytopathogen Verticillium dahliae and Rhizoctonia solani , which caused cotton Verticillium wilt and cotton sore shin, respectively, by secreting antipeptides. To determine the role of the antipeptide in controlling cotton disease, the transposon Tn917 insertional mutagenesis was applied to NCD-2 strain. In this study, B.subtilis NCD-2 was transformed with a plasmid pTV1 carrying Tn917 by protoplast methods. 1500 mutants were screened against V.dahliae and two antibiosis-free mutants were obtained with resistance to erythromycin and lincomycin but susceptible to chloromycetin. The greenhouse experiment on cotton sore shin control with NCD-2 wild stain and antibiosis-free mutants showed that antibiosis of NCD-2 played an important role in controlling this disease. The result suggested that the functional gene of B.subtilis NCD-2 encoding the antipeptide against V. dahliae had a significant function in controlling of cotton sore shin disease.
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