Original£ºCotton Science£¬2006£¬18(6)£º342-346

 

 

Effects of the Salinity Priming on the NaCl Tolerance of
Transgenic Insect Resistant Cotton ( Gossypium hirsutum L.)

LIN Jun£¬SUN Yu-Jiang£¬L¨¹ You-Jun£¬ZHU Shui-Jin*
(Agronomy Department,College of Biotechnology and Agriculture, Zhejiang University, Hangzhou, Zhejiang 310029, China)

Abstract£ºThe effect of seed priming with salts on germination and emergence under NaCl tolerance was studied with two commercial transgenic insect resistant cotton cultivars, CCRI 41 and Z-905, as the materials, and their genetic background cultivar, CCRI 23, as check. The results indicated that it was inhibitive for seed germination and field emergence of the upland cotton when treated by 0.5% NaCl, especially for the transgenic insect resistant cotton cultivars which the germination percentage and field emergence stressed by 0.5% NaCl was much lower than that of their genetic background check. However, priming treatment with a certain concentration salts could alleviate the harmful of the NaCl stress significantly. After the treatment of seed priming, the germination percentage and seedling emergence on the condition of 0.5% NaCl stress increased significantly, and the seed tolerance to the salt stress enhanced greatly. There were some differences in the effect of the seed priming treatments on the seed germination and field emergence, stressed by NaCl, between the transgenic insect resistant cotton and their genetic background one, and the formers, with weak tolerance against NaCl stress, had a better effect than that of their genetic background one without exogenous insect resistant genes. There were some differences among the priming agents, of which Na2SO4 was the best. In addition, there existed interactions between the concentration of the priming agents and the process time. The process time should be longer for the priming agents with lower concentration than that with higher concentration.
Key words£ºsalinity stress£»transgenic insect resistant cotton£»seed priming; seed germination
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