Original：Cotton Science，2007，19(6)：424-430

Molecular Markers of Verticillium Wilt Resistance in Upland Cotton
Gossypium hirsutum L.) Cultivar and Their Effect on
Assisted Phenotypic Selection
WANG Fu-rong¹，LIU Ren-zhong¹，WANG Liu-ming¹，ZHANG Chuan-yun¹，LIU Guo-dong¹，LIU Qin-hong¹，Ma Xiao-bo^1.2，ZHANG Jun¹
（1.Shandong Branch of National Cotton Genetic Improvement Center，Cotton Research Center of Shandong Academy of Agricultural Sciences，Ji＇nan，Shandong 250100, China; 2.College of Life Sciences, Shandong Normal University, Ji＇nan,Shandong 250014,China）

Abstract：Genetic population of F₂ and its corresponding F_2：3 lines derived from a hybridization combination of a Verticillium wilt disease resistance cultivar, of pest-resistant transgenic upland cotton ( Gossypium hirsutum L.), crossed to a Verticillium wilt disease sensible upland cotton line (Luyuan 343), with high fiber quality genomic components introgressed from G. barbadense L., were used to evaluate the resistance of Verticillium wilt disease, and then dissected the resistance genes by SSR markers with the phenotype data of different developmental stage of cotton growth season. qVWR-16-1a , which was detected with the data of the vigorously developmental stage, is located between markers BNL2986 and NAU751 in the fragment of chromosome 16 with 5.73 cM to NAU751, and accounts for 16.53% of the phenotypic variation.The resistance is proved to be from the genotype of resistance parent. Meanwhile, with the data of the late stage of the cotton growth season, three QTLs were detected to be related to the Verticillium wilt resistance. qVWR-16-1b is located in the same interval to qVWR-16-1a with 1.73 cM to the marker NAU751 locus and accounts for 10.27% of the phenotypic variation, while another locus named qVWR-16-2b is located in another interval between BNL1604 and BNL1395, in the same chromosomal fragment with 1.39 cM to BNL1395, and accounts for 10.8% of the phenotype variation. Another QTL named qVWR-2-1b located in the interval between BNL3950 and BNL3971 with only 0.01 cM to BNL 3950 in the fragment of chromosome 2, accounts for 13.78% of the phenotypic variation. Evaluation on the disease resistance of some offspring (F₅) in the breeding population showed that pyramiding the resistant genotypes of marker NAU751 and BNL1395 can significantly improve the Verticillium wilt disease resistance. It was concluded molecular markers could be used to improve Verticillium wilt disease resistance in the process of breeding using the high fiber quality introgression line by assistant selection or pyramiding the resistant genotypes.
Key words：upland cotton (Gossypium hirsutum L.); Verticillium wilt disease resistance; SSR; QTLs; molecular marker assistant selection         [Full Text, 3143KB]