Original：Cotton Science，2006，18(6)：372-378

Cloning and Characterization of an Organ Specific and Pathogen
Responsive Promoter from Cotton ( Gossypium hirsutum L.)
ZHANG Bao-long, YANG Yu-wen, NI Wan-chao, SHEN Xin-lian, SHE Jian-ming,
He Xiao-lan, ZHANG Xiang-gui, XU Ying-jun, YAO Shu
( Institute of Agrobiology Genetics and Physiology , Jiangsu Academy of Agricultural Sciences , Nanjing 210014, China )

Abstract：A promoter region was isolated from the 5 ′ flanking sequence of GHNBS in Gossypium hirsutum by Tail PCR. CAAT-box, TATA-box as well as several pathogen, SA, MeJA and ethylene responsive elements, such as W box, GT-1, MYB and MYBST1 motifs were identified by PLACE analysis while some root specific motifs were also identified in this region. Three enhancer elements including two as-1 and one EECCRCAH1 motifs that were responsible for the strong expression of GUS gene in transgenic plants were located in the far upstream. Fusions of different 5 ′ promoter deletion derivatives with the coding region of the GUS gene were transformed into Arabidopsis . Histochemical localization showed strong staining in roots, phloem of the stem and leaf veins. PGN-1559 and PGN-1117 showed organ specific GUS staining patterns while PGN-476 did not. The fact that GHNBS promoter could enhance the expression level of the GUS gene in transgenic Arabidopsis when treated with SA, ABA, MeJA, ethylene, Fusarium oxyporum and DC3000 showed that there were some regulatory elements in the 5 ′ flanking sequence of GHNBS and which was most possible a pathogen responsive and organ specific promoter.
Key words：promoter; cotton （Gossypium hirsutum L.）; pathogen responsive; organ specific
CLC number：S435.621        Document code：A
Article ID：1002-7807（2006）06-0372-07      [Full Text, 5981]