Original：Cotton Science，2006，8(5)：304-308

Combining Ability and Heterotic Performance of Bt
Transgenic Lines in Cotton ( Gossypium hirsutum L.)
GUO Xiao-ping¹，ZHAO Yuan-ming²，WU Jia-he¹，ZHANG Xian-long^1*，NIE Yi-chun¹
(1.National Key laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070，China；2.Cotton and Oil Crop Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)

Abstract：Five transgenic lines of cotton (Gossypium hirsutum L.) carrying the cry1Ac (Bt，Bacillus thuringiensis ) gene were developed from a cultivar JH321 by independent transformation events. Fifteen F₁ hybrids were obtained from diallel crosses among the five transgenic lines and JH321.When compared with the transformation receptor cultivar JH321, significant variations in agronomic traits including improved yield or yield components were found among these transgenic lines. General combining abilities (GCA) among these transgenic lines varied greatly, and two of these lines with significant positive GCA effects were identified. Heterosis in F₁ hybrids also exhibited significant variations. It was found that the average heterosis of crosses with two transgenic lines was lower than that with only one transgenic line as parent in yield performance. The economic trait improvement for transgenic lines and the heterosis produced between transgenic line and receptor cultivar might be raised from different integration sites of Bt gene.
Key words：upland cotton; combining ability; transgenic lines; Bt genes
CLC number：S562.032     Document code：A
Article ID：1002-7807(2006)05-0304-05     [Full Text,1925]