棉 花 学 报     Cotton Science    2008，20(3)：231-234

Method Comparison of Total RNA Isolation for Young Cotton Buds
SONG Yang¹，WU Qiao-wen^1,2, GUO San-dui^1*
(1.Biotechnology Research Institute，the Chinese Academy of Agriculture Sciences, Beijing 100081;2. Beijing People's Police College, Beijing 102202, China)

Abstract：RNA extraction efficiencies of leaves and young buds from cotton and tobacco were investigated by using hot borate /protease K method and Trizol method, respectively. The result indicated that RNA extracted by hot borate method showed better quality and integrity.
Key words：cotton young bud；RNA extraction；hot borate /Protease K method; Trizol method
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