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棉 花 学 报 Cotton Science 2008，20(2)：99-104
Cloning and Expression Analysis of Two New Genes Encoding F-box Proteins in Cotton ( Gossypium hirsutum L . ) Abstract：In the attempt to elucidate the pivotal function of GA signaling in the initiation and elongation of cotton fiber, two new F-box protein genes were isolated from Gossypium hirsutum for the first time using electronic cloning and RT-PCR methods. They were named GhFB 1 and GhFB 2, respectively and were submitted to NCBI and the GenBank accession were EF419428 and EF503623. The cDNA of GhFB 1 contained 866 bp and encoded a putative protein of 162 aa, while GhFB 2 was 920 bp in length, encoding a predicted protein of 161 aa. The encoding proteins have the typical F-box motif of F-box protein family. Bioinformatics analysis indicated that the identities of the deduced GhFB 1 and GhFB 2 amino acid with Oryza sativa protein, OsGID 2, and Arabidopsis thaliana protein, AtSLY1, are very high. Sequence analysis indicated that these two genes are new members of the F-box protein gene family. The expression patterns of GhFB1 and GhFB2 was analyzed by RT-PCR in cotton. It was demonstrated that these two F-box protein genes were both expressed in root, hypocotyls, leaf, stem and fibers, and the highest level of transcripts was to accumulate in the fibers at the stage of initiation and elongation, suggesting that the two F-box genes, GhFB 1 and GhFB 2, might play important roles in the early stage of fiber development.
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