棉 花 学 报    Cotton Science   2008，20(1)：79-封三

Detection for Persistence of Bt Gene and Bt Insecticidal Proteins in Soil after Multiple Years of Bt Cotton Planting
SHEN Ping^1，2，ZHANG Yong-jun^1*，CHEN Yang¹，WU Kong-ming¹，PENG Yu-fa¹，GUO Yu-yuan¹
(1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2. Technology and Development Center of MOA , Beijing 100026,China )

Abstract:Soil samples were collected from within sixteen fields where insect-resistant transgenic cotton encoding the cry 1A gene had been grown and subsequently incorporated into soil by post-harvest tillage for multiple years. The cry 1A gene sequence fragments and Bt insecticidal protein in these samples (collected after the last season tillage) were explored using PCR amplification and enzyme-linked immunosorbent assay (ELISA) method, respectively. Soil total DNA could be gotten in high quality, but we could not get any PCR amplification- result of cotton internal standard gene or cry 1A gene. The results from ELISA indicated that repeated agricultural using of transgenic Bt cotton expressing Cry 1A insecticidal protein could persist extremely low levels of Bt protein in soil. However, the persistence of Bt protein in soil did not result in accumulation by post-harvest tillage in multiple seasons.
Key words: transgenic Bt cotton; soil; Bt insecticidal protein; persistence
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