棉 花 学 报      Cotton Science      2007，19(6)436-439

Screening of Antagonistic Bacteria Against Verticillium dahliae and Characteristic Analyses of Antagonistic Substance
YUAN Hong-shui¹，MA  Ping²，LI Shu-na¹，HU Ming¹，ZHU Bao-cheng¹
(1.College of Life Science，Agricultural University of Hebei，Baoding 071001，China；2.Institute of Plants Protection，Hebei Academy of Agricultural and Foresty Sciences，Baoding 071000，China)

Abstract：The Bacillus has wide antimicrobial spectrum，which has intensive inhibitory activity against bacteria or fungus caused diseases，thus confer them high potential biological control value. In this paper，the 21 Bacillus strains were fermented in shaking，respectively，the extracellulary metabolic product were precipitated by (NH₄)₂SO₄ salting out. The antifungal activity of the extracted substance were determined through cylinder plate method against Verticillium dahliae Kleb. The result showed that six strains had bigger inhibition zone and had evident antagonistic activity. So these strains were selected to be further tested. Selected six strains which had highs antagonistic activity，their antagonistic substances characteristic，such as stability to chloroform，proteinase K，trypsin，pepsin and heat were analyzed. It was indicated that all the substance were sensitive to chloroform，there had no inhibition zone appearance.
Key words：cotton Verticillium wilt；Verticillium dahliae；antagonistic bacterium；screen
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