棉 花 学 报 Cotton Science 2007，19(4)：273-278
Proteomic Analysis of Cotton Leaf under Verticillium dahliae Stress Abstract：The interaction between cotton and Verticillium dahliae is a field of importance in molecular biology of plant disease resistance. However，there is no report on proteome of cotton under Verticillium dahliae stress. Two-dimensional electrophoresis was employed to analyze the proteome from leaves of cotton glandless variety Yuwu 620 at 24 h，48 h and 72 h，after inoculating Verticillium dahliae. The results showed that there were significant differences between inoculated plants and control in the proteomic map. After infection of the pathogen，a lot of new proteins were induced to express in the cotton seedlings. At the time of 24 h past inoculation，88 new proteins were detected， and at 48 h and 72 h，97 and 101 proteins appeared，respectively. Besides，some of proteins showed intensity differences between inoculated plants and control. Three spots，DB1，DB2 and DB3，were all detected at three stages post inoculation. Analyzing via MALDI-TOF-MS and Mascot software，the three differential expression proteins were partly homologous to DEAD/H box RNA helicase (at 70%)，serine proteinase inhibitor (40%) and ODR-3 protein (85%)，respectively. The protein spot DB1 might have NBS domain and have important function in signal transduction pathway and the immune regulation of host. DB2 belonged to potato proteinase inhibitor I family. It would accumulate in plant as plant was infected by microbes，and could inhibit the proteolytic enzymes of pathogens. DB3 belonged to α subunit of G protein. This protein might combine with receptor to activate some signal transduction pathway. It is likely that they play an important role in the resistance responses to Verticillium dahliae.
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