棉 花 学 报   Cotton Science   2007，19(4)：255-260

Differential Expression Analysis of Aging Cotton Seed ( Gossypium hirsutum ) by cDNA-AFLP
LI Chi^1，2，LU Xin-xiong^2*，CHEN Xiao-ling²，ZHANG Zhi-e²，LIN Feng^1*
(1.Shenyang Agricultural University，Shenyang 110016，China；2.National Key Facility of Crop Gene Resources and Genetic Improvement/ Key Laboratory of Crop Germplasm & Biotechnology (MOA)/Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081，China)

Abstract：Through the cDNA-AFLP analysis of aging cotton seeds ( Gossypium hirsutum ) in four different aging degrees，all different expression fragments were divided into five types. Seven DNA fragments were isolated. By blast search results，one fragment corresponding with CK，16 weeks aging seed and 28 weeks aging seed were homologous with Castanea sativa ribosomal protein L33 mRNA and Arabidopsis thaliana structural constituent of ribosome AT3G55750 mRNA. One fragment corresponding with CK were homologous with oxidoreductase (Arabidopsis thaliana) and 2-nitropropane dioxygenase-related protein-like (Oryza sativa). One of four fragments corresponding with 36 weeks aging seed were homologous with putative tripeptidyl peptidase II (Oryza sativa).one fragment corresponding with 16 weeks aging seed was found no significant homologous sequence in data base.
Key words：cotton(Gossypium hirsutum)；seed aging；cDNA-AFLP；differential expression  [Full Text，3024KB]