棉 花 学 报    Cotton Science   2007，19(4)：248-254

Construction of Suppression Subtractive Hybridization Library with cDNA from Seedling Cotton Induced by Protein Elicitor from Alternaria tenuissima and Analysis of EST
ZHANG Zhi-gang^1，2，3，QIU De-wen^2*，YANG Xiu-fen²，Zeng Hong-mei²，YUAN Jing-jing²，LIU Zheng²，YANG Xiao-ping³，GUAN Chun-yun²
(1.Hunan Agriculture University，Changsha 410128，China；2.Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100081，China；3. Hunan Cotton Research Institute，Changde 415000，China)

Abstract：The gene expression of seedling cotton induced by protein elicitor from Alternaria tenuissima ，the construction of suppression subtractive hybridization (SSH) library with cDNA from seedling cotton，and analysis of EST were described. The results demonstrated that the insertion fragments of SSH library were ranging from 200 bp to 1 kb. 150 clones were randomly picked for PCR and sequencing. After matching the result in GenBank dbEST，104 sequences were obtained. Of them，78 had 48.54%～100% similarity with known genes，which is 75% of the total EST. Six sequences have their homology with the sequences in GenBank with unknown function. 10 have no similarity with the sequences in GenBank，which might be new genes. The function of all the 104 EST was determined by classified being in WEGO and gene ontology. Most of the EST were related to physiological process and cell process，with the number of 48 and 45，respectively. 44 of them related to cell component，30 related to catalytic activity ， 28 related to binding. It is suggested that several pathways were induced when the seedling cotton was treated with protein elicitor，including resistance，cell signaling，transcription，energy metabolism ，et al.
Key words：protein elicitor from Alternaria tenuissima；cotton；suppression subtractive hybridization (SSH)；EST   [Full Text，3845KB]