棉 花 学 报 Cotton Science 2007，19(2)：134-138
Three Methods for Rapid Selection of Transgenic Cotton Plants in Laboratory with Kanamycin as Indirect Marker Abstract：Three methods were developed to rapidly identify Bt-transgenic cotton plants with kanamycin-resistant gene as indirect selective marker. The first method was to culture uncoated seeds on the kanamycin containing medium (kan-medium)，the difference between transgenic and non-transgenic plants was revealed after seeds germinated on the medium. According to the color of the cotyledons，kan-resistant plants were discovered at optimal concentration of 0.75g·L-1. The second method was to paint kanamycin solution (kan-solution) on the cotyledons directly with optimal concentration at 4.0g·L-1，by which after 4-7days，plants with cotyledon normal green were considered as kan-resistant plants. The third one was that a pore was dug on cotyledons and dropped kan-solution in. The best concentration of the kan-solution was 2.0g·L-1. All the plants could be rapidly screened out in 4-7 days after kanamycin treatment by the three methods. The efficacy of above methods for transgenic plant indirect identification were tested using PCR analysis with Bt-gene specific primers，giving a examination ratio of more than 90%. Moreover，all the seeds decorticated could be more easily identified with kanamycin treatments.
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