棉 花 学 报 Cotton Science 2007，19(2)：102-105
The Methods for Examining the Vitality of Cotton Pollen Implanted by Nitrogen Ion Abstract：The vitality of cotton pollen of Sumian 12 cultivar(G. hirsutum L.)implanted by N+ with an energy of 20keV was measured by six methods including in situ pollen germination，Benzidine-α-Naphthol coloring，pollen tube quick germination，FDA fluorescence coloring，TTC coloring and pollen in vitro germination. TTC coloring was not suitable for measuring the vitality of cotton pollen. The other five methods were suitable for measuring natural pollen(CK) vitality with the same result of 98% or so. Pollen tube quick germination couldn′t measure accurately the vitality of pollen being treated in vacuum. Benzidine-α-Naphthol coloring，pollen tube quick germination and pollen in vitro germination were incompetent for measuring the vitality of pollen implanted by nitrogen ion. Only the results of FDA fluorescence coloring and in situ pollen germination were repeatable and suitable for measuring the vitality of pollen implanted by N+. The vacuum treatment had no effect on pollen vitality. It was found that significant negative correlation between ion implantation dosage and pollen vitality exists.
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