棉 花 学 报 Cotton Science 2006，18(5)：284-288
Effects of Heat-shock on Profile and Content of Water-soluble Proteins in Seedling Cotyledon of Three Cotton Cultivars Abstract：Selecting one boll-worm-resistant cotton variety (DP) and two colored cotton varieties (LV and ZH)，cotton seedling growth was heat shock at different growing periods such as germination stage， cotyledon stage and one-leaf stage. The method of heat shock is from 25 ℃ directly moves in 42℃，the relative humidity 65% artificial illumination incubator，deal with two hours. The soluble protein in cotyledon was extracted，and heat shock protein was determined with the SDS-PAGE，electrophoresis gel was discriminated by the GDS-7600 gelatin imagery and the analysis system of English UVP Corporation， molecular weight and the content of every protein was surveyed using fitting protein analysis software. This study shows：Synthesis of heat shock protein had 4 kind of expression after heat shock：the content of soluble protein increased，the content reduced，the content was invariable and others couldn't be detected. Three varieties synthesized the protein with molecular weight of 41.8KD and increased in contents by heat shock in different stages. Furthermore，DP increased in the content for three kinds（41.8KD、36.3KD、31.5KD ），decreased in the content for one kind（22.9KD），un-changed in contents for three kinds（25.6KD、24.4KD 、19.0KD）and detected for ten kinds. LV raised in contents three kinds（41.8KD、24.3KD、19.1KD）of HSPs， two kinds（32.1KD、20.9KD）reduction，three kinds（31.5KD、25.3KD、23.5KD）homology and eight kinds detection. ZH raised in contents six kinds（73.3KD、41.8KD、32.1KD、31.5KD、25.2KD、23.1KD）of HSPs，two kinds（42.6KD、19.1KD）reduction，two kinds（71.5KD、25.2KD）homology and five kinds detection. The possible reason for gain of protein content is that the HSPs combined sequence has a stronger tolerant ability for the high temperature，the synthesis protein is necessary for maintenance；these protein combined sequences are extremely sensitive to the high temperature，which the content of protein is reduction and invariable ，and the synthesis is blocked or the closure in the high temperature. These protein are not very important for vital activities and synthesized protein was also degraded. The possible reason of no-measure contain of other protein is that HSPs gene copying is activated，and most normal protein gene copying is suppressed.
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