棉 花 学 报 Cotton Science 2006，18(5)：259-263
Effect of Solid Substrate Fermentation of C. tropicalis ZD-3 on Detoxification of Cottonseed Meal Abstract：The objectives were to study effects of carbohydrate sources，urea，heat treatment and ferrous sulfate on reduction of gossypol content during solid substrate fermentation of cottonseed meal (CSM). CSM substrate was treated with carbohydrate sources(including glucose，sucrose，maltose and starch)，urea and ferrous sulfate，respectively. Then the mixtures were moistened，(substrate：water was 1∶0.9)，and autoclaved at 112.6 ℃ for 20 min. The treated substrate，100 g in each 500 mL conical flask，was inoculated with 5 mL of C.tropicalis ZD-3，and incubated at 30℃ for 48 h in a 95% relative humidity chamber. After fermentation was complete，assayed the free gossypol (FG)，crude protein (CP) content in CSM substrate. The results showed that CSM substrate supplemented with selected carbohydrate sources was beneficial to fermentation detoxification，of which starch was most effective，the detoxification efficiency up to 91.91%；Heat treatment remarkallely reduced gossypol levels during solid substrate fermentation of CSM，but CSM substrate supplemented with urea could decrease detoxification rate of FG， and was not beneficial to CSM fermentation；Adding ferrous sulfate to CSM substrate could increase detoxification rate of FG significantly，C.tropicalis ZD-3 detoxification efficiency reached up to 94.70%.
|