棉 花 学 报     Cotton Science      2006，18(3)：140-144

Molecular Cloning and Expression Analysis of a Serine/Threonine Protein Kinase Gene in Upland Cotton
YANG Yu-wen, NI Wan-chao^*, ZHANG Bao-long, SHEN Xin-lian,ZHANG Xiang-gui, XU Ying-jun,YAO Shu
(Institute of Agrobiology Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China )

Abstract：The cDNA encoding the full length of the serine/threonine protein kinase like protein of cotton was cloned through RT-PCR. The amino acid sequence encoded by it shows an 82% homology to the ATPK3 of Arabidopsis. This gene was tentatively designated as GhPK1 . The entire coding region of GhPK1 is 1038 bp and encodes a polypeptide of 346 amino acids. Its product may possess one trans-membrane domain in the region of 265 to 270 amino acids and may bind to the membrane of the endoplasmic reticulum. GhPK1 shows a higher expression level in seed and fiber than in other organs. In addition, the expression of GhPK1 is increased by salt induction which indicates GhPK1 may participate in salt-stress reactions.
Key words：upland cotton （Gossypium hirsutum L.）; serine/threonine protein kinase; gene expression ；
            salt induction            [Full Text, 4802KB]