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棉 花 学 报 Cotton Science 2006，18(3)：135-139
Construction of Molecular Linkage Map and QTL Mapping for Earliness in Short-season Cotton Abstract：CCRI 36, a short season cotton variety, and TM-1 were used to create 207 F2 segregating population in Anyang, Henan. Two parents were used to screen 73 polymorphism markers from 518 pairs of SSR primers, 1200 RAPD primers and 153 pairs of SRAP primers. 207 F2 populations were tagged and mapped with 73 polymorphism markers, including 25 SSR, 35 RAPD and 13 SRAP markers. 43 of the informative loci were used in linkage map construction and were assigned into 5 linkage groups. The linkage map was composed of 9 SSR loci, 28 RAPD loci and 6 SRAP loci. The total length was about 1174.0 cM, covering 23.48% of the whole cotton genome. The linkage distance ranged from 11.8 cM to 48.9 cM. The marker number associated with a particular group was from 2 to 23 while 30 loci were not linked to the map. 7.16%-39.73% phenotype variance could be explained in the F2 generation. 12 QTLs were examined, among them 8 QTLs were clustered on the first linkage group according to our study. More than 30% phenotype variance in the F2 segregation population was explained by 3 QTLs relating to the sowing to boll opening period, and the sowing to flowering period and the percent of pre-frost lint, respectively. The linkage map construction for study on short season cotton, QTLs against earliness mapping and clustering haven't been reported before. The results are valuable to further research for earliness MAS of short season cotton.
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