棉 花 学 报 Cotton Science 2006，18(2)：104-108
Study on the Establishment of Plant Regeneration Method in Upland Cotton CCRI 521 Abstract：At present，many regeneration plant systems have been established. But only a few model varieties were fit to those regenerative systems and the genetic types were the main restrictive factor in the process of cotton somatic culture and plant regeneration，and the upland cotton variety CCRI521 with better agronomic properties and tolerance to Verticillium wilt had not been studied. This study obtained cell suspending lines and regenerative plants from upland cotton CCRI521 by the approach of solid and liquid culturing. The results showed that the solid medium MS+1.0 mg·L-1 IBA+0.05 mg·L-1 KT+0.1 mg·L-12，4-D+30g·L-1 glucose was the best for callus induction from hypocotyls in our study. While the liquid medium MS+0.01 mg·L-1 IBA+0.01 mg·L-1 KT+0.005 mg·L-1 2，4-D+1.0g·L-1 glutamine was fit for somatic embryogenesis，somatic embryo could be seen after suspending for 30 days. Then these somatic embryo were transferred into four different MSB media for regenerative plants induction and found the medium MSB+2 g·L-1 glutamine+0.5 g·L-1 asparagines+30 g·L-1 sucrose+6.0 g·L-1 agar was the best for regenerative plants induction in these four different MSB media. And the cycle of plant regeneration was about 6 months in this system.
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