棉 花 学 报 Cotton Science 2006，18(2)：67-73
Cloning and Characterization of A Novel Cotton Fiber Expressed Protein （GhCFE）cDNA Abstract：Through randomly sequencing the cotton fiber cDNA library from line 7235 with elite fiber quality in Gossypium hirsutum L.，a cDNA clone encoding cotton fiber expressed protein (designated as GhCFE， GenBank accession number：DQ073045) was isolated. The full length of this cDNA clone was 1274bp，and its largest open reding frame encoded 332 amino acids. The putative protein of this gene had an isoelectric point of 6.14 and a calculated molecular weight of 37.7KD. It had a signal peptide with 27 amino acid residues in N-terminal. RT-PCR analysis indicated this gene did not express in root or stem but only had a weak expression in leaf，while it could be detected in ovules and fiber cells of different developing periods，especially in elongating fibers. Phylogenetic tree showed GhCFE had high homologies with other CFEs reported before. We cloned GhCFE into the fission yeast ( S. pombe ) vector pREP5N and detected that the gene had no significant effect on elongating cells or thickening cell wall in the transformed yeast. Further ，Sense expression vector containing 35S promoter and both sense and antisense expression vectors containing E6 promoter using pBI121 plasmid were constructed ， and the work of transferring these recombined vectors into Gossypium hirsutum L. by Agrobacterium tumefaciens is ongoing. |