棉花学报 Cotton Science 2006，18(1)：53～57
Advances on Genetic Transformation of Cotton Shoot Apical Meristem via Gene-gun Bombardment Abstract：Genetic transformation of cotton shoot apical meristem via gene-gun bombardment is advantageous over Agrobacterium-meditated transformation and pollen-tube pathway in the genotype-independent and time-consuming. There are many factors affecting the shoot-apex transformation. Before shooting，preparation of explants is important for normal growth. The time of cuttin apical meristem，the remained length of hypocotyls，the usage content of exogenous hormones or active carbon must be considered. After shooting，the selected concentration of antibiotics，the period of media transition and seedling rejuvenated influenced the efficiency of shoot-apex method. Moreover， some physical factors were also essential to transformation， such as the quality of DNA and tungsten or gold powder，bonding of DNA，the parameters and manipulations of particle gun. Some factors were analyzed affecting the transformation efficiency of cotton shoot apical meristem via gene-gun bombardment，and the latest development in cotton genetic transformation of the shoot apical meristem were reviewed. In the paper，The existing problems on the method of transformation were also discussed.
|
||