ÃÞ »¨ ѧ ±¨   Cotton Science  2005£¬17(6)£º353-359

Study of Transgenic Cotton Carrying Bt-CpTI-GNA Genes
WU Xia¹£¬WANG Jiao-juan¹£¬ZHU Zhen²£¬SHANGGUAN Xiao-xia¹£¬ZHANG Lin-shui¹£¬LI Bo¹£¬DU Chun-fang¹£¬LI Yan-e¹
£¨1. Cotton Research Institute£¬Shanxi Academy of Agricultural Sciences£¬Yuncheng£¬Shanxi 044000£¬China£»2. Institute of Genetics and Developmental Biology£¬Chinese Academy of Sciences£¬Beijing 100101£¬China£©

Abstract£ºForeign genes (Bt-CpTI-GNA) resistant to cotton insects were introduced into conventional cotton cultivars by Agrobacterium tumefaciens-mediated transformation. Regenerated plants have been obtained. The results of molecular detection indicated that the foreign genes had been transferred into cotton plants. The results of PCR molecular detection£¬ marker genes and foreign target-gene with insect-resistance were quite regular. Some of their progenies' segregation coincided with Mendel's regularity of segregation£¬but some deviated. This phenomenon may be due to ¡°gene silence¡±.
Key words£ºcotton£»Agorbacterium tumefaciens-mediated transformation£»Bt-CpTI-GNA£»
insectª²resistance£»marker gene            [Full Text, 3906KB]