A Comparision of Isozyme and Protein Patterns of Verticillium dahliae from Cotton in China

Cotton Science 2005，17(5)：289-293

A Comparision of Isozyme and Protein Patterns of Verticillium dahliae from Cotton in China
LI Ya-ning¹, WEI Yan-min^1，2, LIU Da-qun^1*, ZHANG Ting¹
(1.Department of Plant Pathology, College of Plant Protection， Agricultural University of Hebei，Biological Control Centre of Plant Disease and Plant Pests of Hebei Province, Baoding 071001, China; 2.Department of Plant Science and Technology, Beijing Agricultural College, Beijing 102206, China) 

Abstract: Twentythree strains of Verticillium dahliae from various areas of China and one strain from USA were compared by PAGE isozymes and soluble protein patterns analysis to differentiate their virulence to cotton. No difference in malate dehydrogenase (MDH) and esterase isozyme patterns were detected among the strains. The strains were assigned to two PAGE pattern groups based on glutamate dehydrogenase (GDH), peroxidase (POD), superoxide dismutase (SOD), and soluble protein electrophoretic patterns. PAGE Group I consists of twenty nondefoliating strains. Group II includes four defoliating strains.
Key words:Verticillium dahliae; isozyme; soluble protein; PAGE; pathotype
CLC number: S435.621.2 Document code：A
Article ID:1002-7807(2005)05-0289-05