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Cotton Science 2005£¬17(2)£º67-71
Study on Promoting the Rate of Pollen-tube Pathway Transformation in Cotton Abstract: The plant bivalent expression vector pBG121 carried IPT gene was transformed into different cotton cultivars (CCRI 10, CCRI 24, CCRI 36) using pollen-tube pathway method. Through orthogonal experiment, we discussed the effects of genotype, introduction time (after pollination), DNA concentration and EMS concentration on transformation rate in cotton. The orders of importance on the transformation rate was: EMS concentration > acceptor genotype > DNA concentration > introducing time. Correlated with transformation rate, the acceptor genotype and the EMS concentration had significant differences between different treatments; the DNA concentration and the introducing time didn't make significant differences among different treatments. So an optimal condition, in which the transformation rate was promoted, was obtained: the genotype was CCRI24, the DNA concentration was 0.01 g¡¤L-1, the EMS concentration was 0.01 g¡¤L-1, the introducing time was 36 hours after pollination. In order to verify the function of the senescenceª²inhibition chimeric gene and the optimal transformation condition that was screened, we selected CARI10 as the acceptor genotype, introduced 0.01 g¡¤L-1DNA and 0.01 g¡¤L-1 EMS at 36 hours after pollination. Another treatment (CK) was the same as above except EMS . The CK had buffer of the same volume as EMS in optimal condition. The applied result showed that compared with the CK, the transformation rate of optimal condition enhances two times. |
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