Cotton Transformation with Mannose as Selective Agent

棉花学报 Cotton Science 2005，17(1):3-7

Cotton Transformation with Mannose as Selective Agent
YUE Jian-xiong¹，MENG Zhao-hong²，ZHANG Lian-hui³，HAN Shao-jie¹，LIN Qin-tie¹，WANG Wei¹(1.Institute of Engineering and Technology，Guangdong province, Guangzhou 510400, China；2.Institute of Cotton Research，Shanxi Academy of Agricultural Science Yun cheng 044000,China;3.Institute of Molecular Agrobiolog 117604, Singapore)

Abstract: Employing pmi as a selection marker gene and mannose as a selection agent, the GFP gene was transformed into cotton via agrobacterium and regeneration plants were acquired. Transgenic plants were confirmed by southern hybridization and PCR analysis, expression of the GFP gene was showed with fluorescence microscope and West blotting. The concentration and method for employing mannose as selection agent had been studied. The reasonable selection concentration of mannose should be 30-50g·L^-1. The start concentration of mannose in callus-induced medium is less and the concentration increased as callus grow up. Because mannose is not beneficial for embryo differentiation and development, mannose will be replaced with glucose when the callus was transferred to differazation medium.
Key words：cotton; transgene; mannose [Full Text, 2416KB]