棉花学报   Cotton Science      2005，17（5）：289-293

 

我国棉花大丽轮枝菌 (Verticillium dahliae)电泳图谱分析
李亚宁1，魏艳敏1,2， 刘大群1*，张 汀1
（1.河北农业大学植物病理系,河北省农作物病虫害生物防治工程技术中心, 保定 071001；
2.北京农学院植物科学与技术系，北京 102206）

摘要：对24株来自不同地区、作物的不同致病类型的大丽轮枝菌，进行了同工酶和可溶性蛋白电泳图谱分析。结果表明，24株大丽轮枝菌苹果酸脱氢酶酶谱具有种的特异性，酯酶酶谱具有属的特异性。谷氨酸脱氢酶、过氧化物酶、超氧化物歧化酶和可溶性蛋白电泳图谱与病菌的致病类型有关，可将24株大丽轮枝菌分为2群，群Ⅰ包括20个菌株，均属于中等致病力或弱致病力的非落叶型菌系；群Ⅱ包括4株菌，均属于强致病力的落叶型菌系。
关键词：大丽轮枝菌；同工酶；可溶性蛋白；聚丙烯酰胺凝胶电泳；致病类型
中图分类号：S435.621.2     文献标识码：A
文章编号：1002-7807(2005)05-0289-05

References:
［1］ZHANG X Z, Zhang S Q, Chen J D. Identification of Verticillium wilt pathogen of cotton in China ［J］. Acta Phytopathologica Sinica, 1981, 11(3):13-18.
［2］SCHNATHORST W C, Mathre D E. Host range and differentiation of a severe form Verticillium alboatrum in cotton［J］. Phytopathology, 1966, 56:1155-1161.
［3］LU J Y, She C F, Ju L H, et al. Studies on differentiation of the Verticillium dahliae Kleb. in Jiangsu province［J］. Journal of Nanjing Agricultural University, 1983, 83(1):36-43.
［4］YAO Y W, Fu C Z, Wang W L. Preliminary studies on physiological forms of cotton Verticillium wilt fungus［J］. Acta Phytophylacica Sinica , 1982, 9(3):145-148.
［5］WANG Q H, Wu X C, Pan D L. Identification of physiological forms of Verticillium dahliae Kleb. in Shandong province (II)differentiation of physiological forms［J］. Acta Phytopathologica Sinica , 1982, 12(1):19-22.
［6］SHI L Y, Feng J, Wang L M. Physiological differentiation of Verticillium dahliae on cotton in Northern China［J］. Acta Gossypii Sinica , 1997, 9(5):273-280.
［7］XIA Z J, Achar P N, Gu B K. Vegetative compatibility groupings of Verticillium dahliae from cotton in mainland China［J］. European Journal of Plant Pathology, 1998, 104(9):871-876. 
［8］WEI Y M, Zhou Y L. A taxonomic study of Rhodotorula based on isoenzyme patterns［J］. Mycosystema, 1998, 17(1):63-67.
［9］CHU X N, Bai Y M, Yuan J M. A comparative study of electrophoretic patterns of soluble proteins and zymograms from nine strains of Rhizopus［J］. Acta Mycologica Sinica , 1994, 13(2):121-126.
［10］ZERVAKIS G, Labarere J. Taxonomic relationships within the fungal genus Pleurotus as determined by isoelectric focusing analysis of enzyme patterns［J］. Journal of General Microbiology, 1992, 138:635-645.
［11］BONDE M R, Micals J A, Peterson G L. The use of isozyme analysis for the identification of plant pathogenic fungi［J］. Plant Disease, 1993, 77(10):961-968. ［12］BONDE M R, Peterson G L, Maas J L. Isozyme comparisons for identification of Collectotrichum species pathogenic to strawberry ［J］. Phytopathology, 1991, 81(12):1523-1528.