棉花学报   Cotton Science      2005，17（5）：269-274

 

棉铃虫对转Cry1Ac基因棉的抗性遗传及AFLP标记研究
周晓梅，沈晋良*
（南京农业大学植保学院农药科学系，农业部病虫害监测与治理重点开放实验室，南京　210095） 

摘要：抗性遗传分析结果表明，转Cry1Ac基因棉选育抗性棉铃虫（Helicoverpa armigera (Hübner)）对Bt毒素Cry1Ac的抗性遗传方式为不完全隐性，其回交后代（BC）用40 μg·ml-1 Cry1Ac的饲料饲喂5d后，存活虫数∶死亡虫数的比例为1∶1（X2<X20.05）；BtR与AFLP标记EaaMcta02连锁，位于第2条连锁群上EaaMcta02和EaaMcta03两个标记之间，遗传图距分别为10.40 cM和13.90 cM。本研究结果将为制定棉铃虫对转Bt基因棉的抗性监测及抗性治理方案提供一个依据。
关键词：棉铃虫；转Cry1Ac基因棉；抗性遗传；AFLP；分子标记
中图分类号: S562.035      文献标识码 ：A
文章编号：1002-7807（2005）05-0269-06      [全文，5973KB]

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