棉 花 学 报     Cotton Science   2005，17(4)：211-216

 

从棉花ESTs数据库中筛选微卫星标记的初步研究
李华盛1，范术丽2，沈法富1*
(1 山东农业大学农学院，山东泰安 271018；2 中国农科院棉花所 河南安阳 455000)


摘要：利用生物信息学方法在含有94709条棉花EST序列的数据库中进行微卫星标记筛选,共发现微卫星序列4396 个，占整个EST数据库的4.64%。其中双碱基重复序列1282个、三碱基序列2411个，分别占在EST
数据库中发现微卫星序列总数的29.27%和54.8%。根据筛选到的微卫星序列设计并合成引物25对，其中24
对引物有扩增产物，17 对产物条带比较清晰，对这些条带比较清晰的产物做了一系列的分析。
关键词：棉花；表达序列标签；微卫星；通用性分析
中图分类号：S562.035.3     文献标识码：A
文章编号：1002-7807(2005)04-0211-06    [全文，3943KB]


参考文献：
[1]SAHA S, Kanaca M, Jenkins J N, et al .Simple sequence repeats as useful resoures to study transcribed genes of cotton[J].Euphytica,2003,130:355-364.
[2]BASU S,Johnie N J ,Douglas B S. Use of markers for analysis of genetic relationship among Improved cotton cultivars and Its Association with F2 hybrid performance[A] Plant & animal genome IX conference 2001.
[3]NGUYEN T B, Giband M，Brottier P,et al. Wide coverage of the tetraploid cotton genome using newly developed microsatellite markers[J] Theor Appl Genet (2004) 109：167–175
[4]BEZAWADA C, Saha S, Jenkins J N, et al. SSR Marker(s) associated with root knot nematode resistance gene(s) in cotton[J] The journal of cotton science 2003,7：179–184
[5]KARACA M, Saha S, Jenkins J N.Simple sequence repeat (SSR) markers linked to the Ligon lintless (Li(1)) mutant in cotton[J].J Hered. 2002 May-Jun;93(3)：221-4.
[6]ENJAUL I, Sorrells M E, Baum M , et al .Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat[J]. Theor appl genet 2002 104:399–407.
[7]SCHUBERT R，MuellerStarck G,Riegel R. Development of EST-PCR markers and monitoring their intrapopulational genetic variation in Picea bies (L.) Karst[J]. Theor appl genet 2001 103：1223–1231.
[8]ENJAUL I, Sledge M K, Wang L ,et al.Medicago truncatula EST-SSRs reveal crossspecies genetic markers for Medicago spp[J]. Theor appl genet，2004，108:414–4220.
[9]CORDEIRO G M, Casu R, McIntyre C L,et al. Microsatellite markers from sugarcane (Saccharum spp.) ESTs cross transferable to erianthus and sorghum[J]. Plant Sci, 2001 May;160(6)：1115-1123.
[10]沈法富，于元杰，刘凤珍,等.棉花核DNA的提取及其RAPD分析[J]，棉花学报, 1996,8(5)：246-249.
[11]左开井. 转基因抗虫棉Bt基因的遗传效应及定位研究[D].华中农业大学博士研究生学位论文.2000.
[12]GUPTA P K, Rustgi S , Sharma S,et al. Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat [J]. Mol gen genomics(2003) 270： 315–323.
[13]徐 鹏，周令华，田丽萍,等.从中国对虾ESTs中筛选微卫星标记的研究[J].水产学报,2003，27(3)：
13-218.
[14]MORGANTE M, Olivieri A M, PCR amplified microsatellite as markers in plant genetics[J]. Plant J, 1993.3:175-182.