棉 花 学 报    Cotton Science   2010，22（5）：443-447

Physiological Responses of AhCMO Transgenic Cotton Seedlings to Water-Deficit
ZHU Yu-qing^1,2, LUO Zhen¹, ZHANG Hui-jun³, KONG Xiang-qiang¹, CHEN Shou-yi⁴, DONG He-zhong^1*, SUN Xue-zhen^2*
(1. Cotton Research Center,Shandong Academy of Agricultural Sciences,Ji'nan 250100, China; 2.Agronomy College, Shandong Agricultural University,Taian, Shandong 271018, China; 3.Cotton Research Institute,Shanxi Academy of Agricultural Sciences,Yuncheng,Shanxi 044000,China; 4.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)

Abstract: Water-deficit treatment was conducted on two AhCMO transgenic cotton lines (L1 and L2) and a non-transgenic cv. Simian 3(SM3) to study the physiological responses in seedling stage of AhCMO transgenic cotton, by using pots filled with fertile soil in a green house. Soil moistures were maintained 45% and 75% of the soil water capacity as the drought-stressed treatment and the non-stressed control, respectively. No obvious morphological or developmental differences existed between the transgenic lines (L1 and L2) and field plants in the absence of drought stress. The contents of glycine betain, activities of antioxidant enzymes (POD and SOD), the biomass, net photosynthesis rates and leaf chlorophyll content of transgenic AhCMO cotton lines (L1 and L2) were observably higher than those of SM3 at 20 d after stress treatment had been applied. It is suggested that the enhanced accumulation of glycine betain and the increase of the activities of antioxidant enzymes (POD and SOD) improved water-deficit tolerance in transgenic cotton plants.
Key words：cotton; AhCMO gene; water-deficit; physiological response    [Full Text, 396KB]