ÃÞ »¨ ѧ ±¨      Cotton Science     2009£¬21(6)£º503-507

 

 

 

Soil Microbial Community Structure Change Analysis of Transgenic Bt Cotton Based on Phospholipid Fatty Acid Method
LI Yong-shan£¬FAN Qiao-lan£¬LI Yan-e Geng£¬CHAI Yong-feng£¬ZHANG Dong-mei£¬LI yan-e
(Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng, Shanxi 044000, China)

Abstract£ºThe experiment was conducted to evaluate the effects of transgenic Bt cotton on soil microbial community in 2006 under the field based on phospholipid fatty acid (PFLA) method.Three transgenic Bt cotton lines ( JM26,JM44 and CCRI41) and one non-Bt cotton line were used.The results showed transgenic Bt cotton increased amount of PFLA for soil microbe.Bt-cotton soils were characterized by the predominance of actinomycetes,fungi and eukaryotes in comparison with non-Bt cotton soil.In contrast,the microbial community was characterized by the predominance of Gram-positive bacteria in non-Bt cotton soil.Soil microbial community were characterized by the predominance of protoza and Gram-negative bacteria for Bt-cotton soils in square growth stage,and for non-Bt cotton soil in the open boll stage.PFLA anlysis was accepted as indicator for risk evaluation of transgenic Bt cotton on soil microbial community.
Key words£ºtransgenic Bt cotton; soil microbe; community structure;PLFA    [Full Text, 2031KB]