ÃÞ »¨ ѧ ±¨           Cotton Science     2009£¬21(6)£º468-473

 

 

RAPD Analysis of Gossypium bickii and Its Hybrid Descendents
QU Yun-fang1£¬HUANG Jin-ling1*£¬YI Zhi-ben2£¬LI Bing-lin1
£¨1.Dept of Agron, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 2.North University of China, Taiyuan, Shanxi 030051, China£©

Abstract£ºThe interspecific hybrids were derived from £Û(Gossypium arboreum¡ÁGossypium bickii)¡ÁGossypium hirsutum £Ý,(G.arboreum¡ÁG.bickii)¡ÁGossypium barbadense£Ý,£Û(G.arboreum¡ÁG.bickii)¡Á(G.hirsutum¡ÁGossypium mustelinum)£Ý,(G.arboreum¡ÁG.bickii)¡Á(G.hirsutum¡ÁGossypium tomentosum)£Ýafter doubling F1 of ( G. arboreum ¡Á G.bickii ). These hybrids and their parents were analyzed by RAPD. The results showed that£º£¨1£©There were 127 amplified DNA bands from 15 primers, 102 bands (80.3 %) were polymorphic£»(2) Parents bands and specific bands of hybrids were detected in hybrids; (3) The cluster analysis of materials was studied by group-average method from SAS software. Cluster analysis revealed that these materials were divided into 3 groups. All the results verified the authenticity of hybrids.
Key words£ºGossypium bickii£»hybrid descendents£»RAPD    [Full Text, 3262]