棉 花 学 报        Cotton Science      2009，21(5)：420-426

Analysis of Genetic Diversity in Cotton Germplasm by TRAP Markers
MIAO Pei-ming¹, FAN Ling^1*, SHI Wei-jun², Wumaierjiang², XU Li-min²，MA Jun²
(1.Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China；2.Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China)

Abstract：The genetic diversity among 65 lines of cotton germplasm was analyzed by TRAP (Target Region Amplification Polymorphism) markers. From 100 pair of primers, 10 pairs of polymorphic primers were selected. A total of 252 bands were amplified from 10 pairs of primers by TRAP-PCR, of which 210 bands (about 83.33%) were polymorphic. The 65 lines of cotton germplasm were clustered into six groups at GS (genetic similarities) = 0.8431 and the link map was set up by UPMGA method in NTSYSpc 2.1(numerical taxonomy and multivariate analysis system).The results showed that the germplasm clusters had certain relationship with the germplasm origins and the morphologic characters. The results suggested that the new type of molecular maker method, TRAP, was suitable for genetic identification, cluster of cotton germplasm.
Key words：cotton; germplasm; genetic diversity; TRAP     [Full Text, 4226KB]