ÃÞ »¨ ѧ ±¨ Cotton Science 2009£¬21(5)£º339-345
Construction of SSH Library on Root System of Salinity-Tolerance Variety ( G. hirsutum L.) under the Stress of Salinity Abstract£ºA SSH library from the salt tolerant upland cotton cultivar, CCRI 35, was constructed using suppression subtractive hybridization (SSH) and pGEM-T Easy Vector under salt (0.4% of NaCl) stress at the first, third, fifth and seventh day after the beginning of salination. The library was evaluated to be of good quality with high efficiency of SSH. Total 200 positive clones were randomly selected and then sequenced, and 160 high quality sequences were obtained. According to BLAST screening and functional annotation, there were 62 unigenes (38.8%) without or only with weak similarities to the existent proteins in the NR database from NCBI and 42 unigenes £¨26.2%£© significantly matched with hypothetical protein. While the unigenes assigned to stress-tolerance, metabolism, signaling conduction and transcription regulation accounted for 8.8%, 6.9%, 6.3% and 5.0%, respectively. The functions of these unigenes under salt stress were discussed.
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