棉 花 学 报      Cotton Science 2009，21(4)：265-270

The Anther Development Research on Double Recessiveness Nuclear Male-sterile Line ms₅ms₆ of Gossypium hirsutum L.
DOU Li-ping¹， WANG Qing-ya²，TANG Can-ming^1*
(1.Agronomy College，Nanjing Agricultural University，Nanjing 210095，China；2.Life Science College，Nanjing Agricultural University，Nanjing 210095，China)

Abstract：The double recessiveness nuclear male-sterile line ms₅ms₆of upland cotton ( Gossypium hirsutum L.) has been used in cotton seed production, but there is no report on its abortion stage and character. The result of embryology study showed the abortion stages were the development periods of pollen mother cells and microspore, which was the main abortion stage observed using paraffin slice technique under light microscope. The abortion characters of the double recessiveness nuclear male-sterile line ms₅ms₆of upland cotton were: low frequency of nucleolus penetration through nucleus envelop and cell wall and part of pollen mother cells appeared in the shape of lunule; microspore adhesion and few of pentads were found during the periods of microspore; there was no spinescent protuberance on the pollen wall, finally the microspore appeared abnormal and breakdown; there was no difference between fertile anther and sterile anther about the tapetum but the function of tapetum producing corpus callosum is abnormal.
Key words：upland cotton；nuclear male-sterile line；pollen mother cell；microspore；anther
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