棉 花 学 报      Cotton Science 2009，21(2)：94-99

Optimization of Extraction Technology Condition of Gossypol from Cottonseed Meals Using Response Surface Methodology
WU Yuan-jin，WANG Cheng-ming^*，WU Mou-cheng
( MOA Key Laboratory of Food Safety Evaluation, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China )

Abstract： Optimizing extraction technology of gossypol from cottonseed meals with the hydrochloric acid and 80% ethanol mixed solution by Response Surface Methodology's Box-Behnken mode was studied. A quadratic equations model for gossypol extraction was built and effect of main factors and their corresponding relationships were obtained. The optimal extraction conditions of gossypol from cottonseed meals as follows: extraction temperature 60 ℃ , volume of extractant 26 mL·g^-1, hydrochloric acid's concentration 1 mol·L^-1, extraction time 3.6 h,extracting times was 1, the yield of gossypol from cottonseed meals by hydrochloric acid and 80% ethanol mixed solution was 8.80 mg·g^-1.
Key words：gossypol; extraction; response surface methodology      [Full Text, 2371KB]