ÃÞ »¨ ѧ ±¨ Cotton Science 2009£¬21(2)£º89-93
Studies on the Inhibition of Plant Derived Protein CkND Against Verticillium dahliae and Its Drought Resistance Abstract£ºProtein CkND, a novel disease-resistant and drought-resistant protein, was extracted form the roots of Cynanchum Komarovii by method of proteomics. In this study, we tested the fungicidal activities and resistance of protein CkND against Verticillium dahliae. The results showed that short protein CkND may have a good inhibition on the spore germination and mycelial growth of Verticillium dahliae . In vitro test suggested that 100 mg¡¤L-1 of protein CkND on mycelial growth at 24 h, 48 h, 72 h was 79.53%, 83.01% and 87.50£¥£¬ respectively£¬and 35 mg¡¤L-1 of protein CkND on spore germination of Verticillium dahliae was 100%. In vivo test showed that protein CkND had good inhibitory effects against Verticillium dahliae. In addition, the CkND gene was constructed into the downstream of the CaMV35S promoter of the pCAMBIA1304 expression vector and transfer of pCAMBIA1304-CkND was transferred into Arabidopsis-thaliana mediated by Agrobacterium tumefaciens . We take 33 pCAMBIA1304-CkND T2 transgenic plants and 21 pCAMBIA1304 T2 transgenic plants into drought-resistant experiments at the same time. The results showed that 14 of the pCAMBIA1304 T2 transgenic plants died, but 24 pCAMBIA1304-CkND T2 transgenic plants survived. The leaves kept green, the plant growed well, roots and fibrous roots developed strongly, and the number of fibrous roots increased obviously. The drought-resistant of pCAMBIA1304- CkND transgenic plants increased 30%, which obviously enhance the drought-tolerance of plants.
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