棉 花 学 报   Cotton Science 2008，20(6)：425-430

Analysis of the Inducing Responsiveness in Cotton Against Protein Elicitor from Alternaria tenuissima Using Two-dimensional Electrophoresis and Mass Spectrometry
ZHANG Zhi-gang^1，2，3，QIU De-wen^3*，GUAN Chun-yun², YANG Xiu-fen³，XIAO Li-yi¹，YANG Xiao-ping¹
(1.Hu′nan Cotton Research Institute，Changde，Hu′nan,415101 China; 2.Hu′nan Agriculture University，Changsha 410128; 3.Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100081, China)

Abstract：Proteins extracted from the cotton seedling induced by protein elicitor from Alternaria tenuissima were separated by two dimensional polyacrylamide gel electrophoresis (2-DE) and Mass Spectrometry. Reference maps were obtained with a good reproducibility. After analyzing with 2-DE software, approximately 600 proteins per 2-DE gel were isolated with molecular weights ranging from 10 kD to 100 kD and PI ranging from 3 to 10. About 53 spots expressed differently, with 31 at a higher level and 22 at a lower level. Seven stronger expressed proteins of high levels observed in a secondary PEAT gel were excised and identified using peptide mass fin-reprinting and matrix-assisted laser desorption ionization time -0f -flight mass spectrometry. Four identified proteins and their putative functions were presented. Three of them, spot no. 1,3,6 were identified as ribulose-1，5-bisphosphate carboxylase,and spot no. Seven was identified as S-adenosyl-L-methioine sythetase.
Key words：PETA；2-DE；MALDI-TOF-MS；cotton     [Full Text. 2990KB]