棉 花 学 报      Cotton Science    2008,20(3):192-197

 

 

Primary QTL Mapping of Upland Cotton RIL CRI-G6 by SSR Marker
ZHANG Xian-liang,WANG Kun-bo*,SONG Guo-li,LIU Fang,LI Shao-hui,WANG Chun-ying,ZHANG Xiang-di,WANG Yu-hong
(Cotton Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture, Anyang, Henan, 455000, China)

Abstract:Two typical upland cotton (Acala1517 and Dezhou 047) were applied to construction of intraspecific recombinant inbred lines (RIL). The agronomic trait of RIL were studied. The trait difference of two parents were significant or extremely significant based on field investigation. The agronomic trait performances of the RIL completely matched the heredity characters of multi-loci quantitative trait. The polymorphism screening result of molecular marker indicated that the genetic relationship of two parents was near. So it was difficult to construct the high density linkage map using the RIL. But the RIL should raise the accuracy when it was applied to the gene location study. 51 SSR markers were assigned into 15 linkage groups. The total length of the linkage map is about 504.05 cM, covering 10.08% of the whole cotton genome. 15 QTLs were detected based on two years agronomic trait data, including three concerned with earliness, seven with fiber quality and five with yield trait. Seven SSR marker tightly linked with the QTL site were valuable to the marker assisted breeding.
Key words:upland cotton;RIL;QTL;SSR     [Full Text,2566KB]